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Experiment and Guide for Medical Biology
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Experiment and Guide for Medical Biology

  • 作者:侯燕芝
  • 出版社:高等教育出版社
  • ISBN:9787040334333
  • 出版日期:2012年01月01日
  • 页数:108
  • 定价:¥20.00
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    内容提要
    《医学生物学实验(英文版)》内容简介:This manual is designed to be used in a course of experiments in medical biology. It will serve mainly the needs of international students of clinical medical major. It will serve any student who wishes to leam both major principles and modern techniques of medical biology.
    By using this manual,students will develop laboratory skills,become familiar with the methods of science,and learn important concepts of experiments in medical biology. The manual provides students with an
    目录
    Chapter1 Introduction to the Medical Biology Laboratory
    11 Objectives of a Teaching Medical Biology Laboratory
    12 Laboratory Rules
    13 Laboratory Safety
    14 Common Laboratory Equipments
    15 Experimental Methods in Medical Biology
    16 Evaluation of Experimental Data and Laboratory Reports

    Chapter 2 Experiments in Medical Biology
    Experiment 1 The Use of Glass Pipets and Pipettors N
    Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins
    Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie BriHiant Blue As Tay NN
    Experunent 4Quantitative Determination of Proteins by Folin-phenol Assay
    Experiment 5 Determination oF Proteins by UV Absorption
    Experiment 6 Separation of Arrmio Acid MixtIHes by Ion-exchange Chromatography
    Experiment 7 Separation of Serum y-globulins by Ion-exchange Chromatography
    Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane
    Electrophoresis
    Experiment 9 Separation and Purity Determination of Serum y-globulins by SDS-polyacrylamide Gel Electrophoresis
    Experiment 10 Determination of Serum Glutamicpyruvic Transaminase Activity
    Experiment 11 Determination of Serum Urea Nitrogen Content
    Experiment 12 Determination of Blood Glucose Concentration
    Experiment 13 Subculture of Adhesion Cells NN
    Experiment 14 Counting and Determination of Live/Dead Cells
    Experiment 15 Preparation of Cell Samples for Observation of Cell Morphologies
    Experiment 16 Mitosis and Meiosis of Cells
    Experiment 17 Intracellular Distribution of Biomacromolecules
    Experiment 18 Isolation of Subcellular Components
    Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells
    Experiment 20 Banding Pattern of Human Chromosomes
    Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells
    Experiment 22 Isolation,Amplification and Identification of the pactin Gene Fragment from Mouse Liver Cells
    Experiment 23 Purification of Target DNA and Generation of Recombinant DNA
    Experiment 24 Transformation and Screening of Recombinant DNA
    Experiment 25 Determination of Serum Proteins Using Western Blotting

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